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基礎信息Product information

產品名稱：DIG High Prime DNA Labeling and Detection Starter Kit I

產品型號：Roche 11745832910

更新時間：2016-12-27

產品簡介：

DIG High Prime DNA Labeling and Detection Starter Kit II|DNA高效地高辛標記及檢測試劑盒II

產品特性Product characteristics

DIG High Prime DNA Labeling and Detection Starter Kit I

DIG High Prime DNA Labeling and Detection Starter Kit I|DNA高效地高辛標記及檢測試劑盒II|地高辛標記試劑盒|高效地高辛標記試劑盒|羅氏地高辛標記試劑盒


DNA高效地高辛標記檢測試劑盒I（羅氏Roche 貨號：11745832910）

DIG-High Prime DNA Labeling and Detection Starter Kit I

Kit for color detection with NBT/BCIP.


1 kit for 12 labeling reactions and detection of 24 blots 10 ng to 3 µg DNA per assay, blots of 100 cm2

Application

DIG-High Prime labeled probes can be used in Southern-, northern- and dot-blot analysis, as well as colony and plaque hybridizations. This kit offers random-primed labeling of DNA templates with DIG-11-dUTP, alkali-labile, and color detection of the DIG-labeled hybrids. This kit was assembled with convenience in mind, offering a ready-made blocking solution, combined stock solution of NBT/ BCIP, and DIG Easy Hyb granules. The DIG-High Prime mixture includes stabilized Klenow enzyme, nucleotides, primers, and reaction buffer, all in one convenient reagent.

Benefits

The High Prime method of labeling offers many benefits. Plasmid, cosmid, and lambda phage DNA have all been used as templates. We have achieved equal efficiency when labeling 10 ng to 3 µg of template DNA, which may be linear or supercoiled.

Product Description

Sensitivity and specificity: The standard assay uses 1 µg of linearized pBR328. It is usual to expect 0.8 µg labeled DNA after 1 hour of labeling, and 2.3 µg labeled DNA after a 20-hour reaction. In a dot-blot hybridization, 0.1 pg of homologous DNA is detectable after a 16-hour color development (probe concentrations at 20 ng/ml). A single-copy human gene (tPA) is detectable in a Southern blot, loading 1 µg of digested placenta DNA.

Contents

  1. DIG-High Prime, 5x conc., 50 µl
  2. DIG-labeled Control DNA, 20 µl, (5 µg/ml), pBR328 (linearized with Bam HI)
  3. DNA Dilution Buffer, 3 x 1 ml
  4. Anti-digoxigenin-AP Conjugate, 100 µl
  5. NBT/BCIP Stock Solution, conc., 6 x 1 ml
  6. Blocking Solution, 10x conc., 4 x 100 ml
  7. DIG Easy Hyb Granules, 4 x 100 ml

 

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