服務熱線
021-60490116
021-51875103
全國統一服務熱線：
021-51875103產品中心您當前的位置：首頁 > 產品中心 > 進口試劑 > 進口品牌 > Roche 11585614910DIG High Prime DNA Labeling and Detection Starter Kit II
基礎信息Product information
產品名稱：DIG High Prime DNA Labeling and Detection Starter Kit II
產品型號：Roche 11585614910
更新時間：2016-12-27
產品簡介：
DIG High Prime DNA Labeling and Detection Starter Kit II|DNA高效地高辛標記及檢測試劑盒II
產品特性Product characteristics
DIG High Prime DNA Labeling and Detection Starter Kit II
DIG High Prime DNA Labeling and Detection Starter Kit II|DNA高效地高辛標記及檢測試劑盒II|地高辛標記試劑盒|高效地高辛標記試劑盒|羅氏地高辛標記試劑盒
DNA高效地高辛標記及檢測試劑盒II （Roche 貨號： 11585614910）
DIG-High Prime DNA Labeling and Detection Starter Kit II
1 kit for 12 labeling reactions and detection of 24 blots 10 ng to 3 μg per assay, blots of 100 cm2
Application
DIG-High Prime labeled probes can be used in northern, Southern-, and dot-blot analysis, as well as colony and plaque hybridizations. This kit offers random-primed labeling of DNA templates with DIG-11-dUTP, alkali-labile, and chemiluminescent detection of the DIG-labeled hybrids. This kit was assembled with convenience in mind, offering ready-to-use CSPD supplied with a dripping device for easy application, ready-made blocking solution, and DIG Easy Hyb granules. The DIG-High Prime mixture includes stabilized Klenow enzyme, nucleotides, primers, and reaction buffer, all in one convenient reagent.
Benefits
The High Prime method of labeling offers many benefits: in addition to the optimized labeling efficiency, plasmid, cosmid, and phage DNA, linearized or supercoiled can be used as templates, yielding the same labeling efficiency.
Product Description
Sensitivity and specificity: Standard assay uses 1 µg of linearized pBR328. It is usual to expect 0.8 µg labeled DNA after 1 h of labeling, and 2.3 µg labeled DNA after 20 h reaction. In a dot blot hybridization, 0.03 pg of homologous DNA is detectable with chemiluminescence (probe concentrations at 20 ng/ml). A single copy human gene (t-PA) is detectable in a Southern blot, loading 1 µg of digested placenta DNA.
Contents
- INNOVEXBIO代理商產品之NB335-60-60ML Fc Receptor Blocker – Azide-Free
- phoreus biotech代理商產品之轉染試劑BAPtofect-25 5mg kit
- baseclick代理產品之BCFA-246-5mg，Tri-β-GalNAc-PEG3-Azide
- medilumine代理商之產品HDVC-121，Fenestra HDVC動物CT造影劑
- Bio/Data Corporation代理商之產品105997 UPTT™ REAGENT
- tulip biolabs代理 2302-1mg，Af1521 Macrodomain Affinity Resin